Composite

Part:BBa_K3356007:Design

Designed by: Luke Weir   Group: iGEM20_Nottingham   (2020-10-27)


Ketone Production Operon DBHBb under the control of the inducible PLAC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 411
    Illegal XbaI site found at 1420
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 411
    Illegal BglII site found at 471
    Illegal BamHI site found at 3527
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 411
    Illegal XbaI site found at 1420
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 411
    Illegal XbaI site found at 1420
    Illegal AgeI site found at 2269
    Illegal AgeI site found at 2941
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3130
    Illegal SapI.rc site found at 3142


Design Notes

TBC


Source

TBC

References